University of Illinois Urbana-Champaign

Investigation of the Chemiluminescent Reaction Between Lucigenin and Organic Reductants and Its Application as a Detector for High Pressure Liquid Chromatography

Veazey, Robert Lawrence

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Description

Title
Investigation of the Chemiluminescent Reaction Between Lucigenin and Organic Reductants and Its Application as a Detector for High Pressure Liquid Chromatography
Author(s)
Veazey, Robert Lawrence
Issue Date
1980
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Analytical
Language
eng
Abstract
  • The reaction of basic lucigenin (N,N'-dimethyl-9,9'-biacridinium dinitrate) with organic reductants was investigated as an analytical tool. Investigation of this reaction was carried out in three distinct steps: (1) stopped flow investigation of the reaction parameters; (2) development of a flow injection system for investigation, specifically the development of a chemiluminescence detector for HPLC, and; (3) application of the chemiluminescence detector to the determination of ascorbic acid and dehydroascorbic acid.
  • The stopped flow data indicated that simple one-component samples of ascorbic acid, creatinine, uric acid, glutathione, glucuronic acid, lactose, and glucose can be quantitatively measured within their important clinical concentration ranges. Two component mixtures of glucose and ascorbic acid were observed to exhibit a response which was a simple addition of their individual responses.
  • Continuous flow instrumentation was designed, built, and tested. Reagent delivery and flow cell design were shown to play an important role in achieving reproducible and reliable data.
  • Ascorbic acid and dehydroascorbic acid were quantitated in the effluent of an HPLC, utilizing the chemiluminescence detector. Vitamin C tablets were assayed using the technique, yielding data that agreed to within 1-5% of titration results. A separation of glucose, glucuronic acid, uric acid, and ascorbic acid on an anion exchange column, at concentrations within their clinical ranges, was detected via the chemiluminescence method.
Graduation Semester
1980
Type of Resource
text
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http://hdl.handle.net/2142/67234

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