University of Illinois Urbana-Champaign

IL-4 Western Blot Analysis of High Fat Diet and Control Diet Sprague Dawley Rats

Mitchell, Emmanuel; Kriska, Adam; Pan, Yuan-Xiang

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https://hdl.handle.net/2142/66349

Description

Title
IL-4 Western Blot Analysis of High Fat Diet and Control Diet Sprague Dawley Rats
Author(s)
  • Mitchell, Emmanuel
  • Kriska, Adam
  • Pan, Yuan-Xiang
Issue Date
2014
Keyword(s)
  • rats
  • maternal programming
  • epigenetics
  • inflammation
  • cytokines
Abstract
The Lowry Protein Assay is a fundamental process in the ultimate procedure of Western Blotting; it is used to determine the protein content of a solution, which is essential in calculating the necessary concentration of antibodies. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels were prepared with a 12% resolving gel and 5% stacking gel. After running the SDS-PAGE, we performed a western blot analysis on our samples. Western blot results showed that there was a significant difference between IL-4 expression in our two different animal experimental groups. Animals with a maternal high fat diet showed increased expression of IL-4, but animals with a maternal control diet showed lower expressions of IL-4. Methylated DNA immunoprecipitation also confirmed our results and showed a region of hypermethylation upstream of the IL-4 gene in the control group.
Type of Resource
text
Language
en
Permalink
http://hdl.handle.net/2142/66349
Copyright and License Information
Copyright 2014 Emmanuel Mitchell, Adam Kriska, Yuan-Xiang Pan

Owning Collections

i-ACES - Vol. 1, no. 1 2014 PRIMARY