IL-4 Western Blot Analysis of High Fat Diet and Control Diet Sprague Dawley Rats

Mitchell, Emmanuel; Kriska, Adam; Pan, Yuan-Xiang

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https://hdl.handle.net/2142/66349 Copy

Description

Title

IL-4 Western Blot Analysis of High Fat Diet and Control Diet Sprague Dawley Rats

Author(s)

• Mitchell, Emmanuel
• Kriska, Adam
• Pan, Yuan-Xiang

Issue Date

2014

Keyword(s)

• rats
• maternal programming
• epigenetics
• inflammation
• cytokines

Abstract

The Lowry Protein Assay is a fundamental process in the ultimate procedure of Western Blotting; it is used to determine the protein content of a solution, which is essential in calculating the necessary concentration of antibodies. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels were prepared with a 12% resolving gel and 5% stacking gel. After running the SDS-PAGE, we performed a western blot analysis on our samples. Western blot results showed that there was a significant difference between IL-4 expression in our two different animal experimental groups. Animals with a maternal high fat diet showed increased expression of IL-4, but animals with a maternal control diet showed lower expressions of IL-4. Methylated DNA immunoprecipitation also confirmed our results and showed a region of hypermethylation upstream of the IL-4 gene in the control group.

Publisher

College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign

Type of Resource

text

Language

en

Permalink

http://hdl.handle.net/2142/66349

Copyright and License Information

Copyright 2014 Emmanuel Mitchell, Adam Kriska, Yuan-Xiang Pan

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