Squalene Production using Saccharomyces cerevisiae

Thompson, Ariana; Kwak, Suryang; Jin, Yong-Su

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https://hdl.handle.net/2142/66264 Copy

Description

Title

Squalene Production using Saccharomyces cerevisiae

Author(s)

• Thompson, Ariana
• Kwak, Suryang
• Jin, Yong-Su

Issue Date

2014

Keyword(s)

• saccharomyces cerevisiae
• 3-hydroxy-3-methylglutaryl-coenzyme-A reductase

Abstract

Squalene is a valuable natural substance found in nearly all organisms, and can be produced by the yeast Saccharomyces cerevisiae. The key regulatory enzyme in squalene biosynthesis is 3-hydroxy-3-methylglutaryl-coenzyme-A (HMG-CoA) reductase (Hmg1). To overcome further down regulation of Hmg1, a truncated Hmg1 (tHmg1), an enzyme which has the catalytic domain of the Hmg1 only, and is not regulated by intermediates, was overexpressed instead of the original enzyme. Because of its strong and fast glycolysis, S. cerevisiae converts substantial portions of glucose into ethanol rather than into the squalene. In order to overcome this problem, the slower glucose assimilating S. cerevisiae (SK2) was employed for the overexpression of the tHmg1 enzyme. Accumulated squalene in the SK2 cells were extracted by using the alcohol/KOH method. The SK2 strain with the overexpression of tHMG1 produced about 30 times more squalene than the strain without the tHMG1. Additionally, the Methanol/KOH combination for squalene extraction showed better squalene recovery than ethanol/KOH combination.

Type of Resource

text

Language

en

Permalink

http://hdl.handle.net/2142/66264

Copyright and License Information

Copyright 2014 Ariana Thompson, Suryang Kwak, Yong-Su Jin

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