University of Illinois Urbana-Champaign

SfN 2014 Poster: Layer-specific differences in the mouse auditory corticocollicular pathway: an anatomical study

Yudintsev, Georgiy; Lesicko, Alexandria, M.H.; Llano, Daniel A.

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https://hdl.handle.net/2142/55879

Description

Title
SfN 2014 Poster: Layer-specific differences in the mouse auditory corticocollicular pathway: an anatomical study
Author(s)
  • Yudintsev, Georgiy
  • Lesicko, Alexandria, M.H.
  • Llano, Daniel A.
Issue Date
2014-11-19
Keyword(s)
Systems Neuroscience
Abstract
The auditory corticocollicular (CC) projection has recently garnered much attention due to its ability to alter response properties of cells in the inferior colliculus (IC). However, the basic anatomical organization of this pathway still remains poorly understood. CC cells emanate from two distinct bands of the auditory cortex – layer 5 and deep layer 6 – and differ in their firing properties and cellular morphology. In the present study, we sought to characterize potential anatomical differences between layer 5 and layer 6 CC cells using retrograde and anterograde tract-tracing techniques. We injected fluorogold, a retrograde tracer, into the IC of the mouse and used Neurolucida to create 3-D reconstructions of the cellular distribution of CC cells in layer 5 and 6 of the auditory cortex (AC). Reconstructions were aligned with acoustically-driven response maps obtained with transcranial flavoprotein autofluorescence imaging. We found heterogeneity in the distributions of the two layers, such that there exists a rostro-ventral area of the AC in which layer 6 corticocollicular cells were more dominant than layer 5 corticocollicular cells. To investigate potential differences in terminal size and projection patterns between layer 5 and layer 6 CC neurons, we injected dextran amine anterograde tracers into either layer 5 or layer 6 of the AC of mice. We found projections from both layer 5 and layer 6 in all subdivisions of the IC. On average, layer 5 axons were thicker than layer 6 axons and their terminals were larger and had a wider distribution of lengths than layer 6 terminals. These data further suggest that the layer 5 and layer 6 CC projections differ in their basic organization and, therefore, could serve distinct functions in auditory processing.
Type of Resource
image
Language
en
Permalink
http://hdl.handle.net/2142/55879
Copyright and License Information
Copyright to Georgiy Yudintsev

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