Fluorescence switch for selectively sensing copper and histidine in both vitro and living cells

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https://hdl.handle.net/2142/55868

Title: Fluorescence switch for selectively sensing copper and histidine in both vitro and living cells
Author(s): Wang, Xiaojing
Issue Date: 2014-06-17
Keyword(s): Biology, natural substances
Abstract: \begin{wrapfigure}{r}{0pt} \includegraphics[scale=0.4]{wxj.eps} \end{wrapfigure} One new synthetic probes for the detection of copper and Histidine in both vitro and living cells. In the absence of metal ions, the new established probes exhibits comparable fluorescence to that of free FITC. In the presence of metal ions, probes selectively coordinates with Cu$^{2+}$, causing its fluorescence emission quenched via photoinduced electron transfer. Interestingly, as-formed complex selectively responds to $_{L}$-His among the 20 natural AAs by turning its fluorescence on. Using this dualfunctional probe, we also sequentially imaged Cu$^{2+}$ and $_{L}$-His in living cells. Our new probe could be applied for not only environment monitoring or biomolecule detections, but also disease diagnoses in the near future.
Publisher: International Symposium on Molecular Spectroscopy
Type of Resource: text
Language: en
Permalink: http://hdl.handle.net/2142/55868
Copyright and License Information: Copyright 2014 by the authors. Licensed under a Creative Commons Attribution 4.0 International License. http://creativecommons.org/licenses/by/4.0/