Bioenergetics and diffusion in the crowded milieu of living cells

Heikal, Ahmed A.

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https://hdl.handle.net/2142/55863 Copy

Description

Title

Bioenergetics and diffusion in the crowded milieu of living cells

Author(s)

Heikal, Ahmed A.

Issue Date

2014-06-17

Keyword(s)

Mini-symposium: Spectroscopy in Kinetics and Dynamics

Abstract

Intracellular nicotinamide adenine dinucleotide (NADH) is a key cofactor in energy metabolism pathways and a myriad of oxidation-reduction reactions in living cells. The crowded milieu of these cells with organelles and macromolecules influences many biological processes such as biomolecular diffusion, protein-protein and protein-substrate interactions, and protein folding. In this contribution, I will highlight our recent findings on the role of macromolecular crowding on biochemical reaction between NADH and selected dehydrogenases in both living cells and in controlled macromolecules-rich environment. In addition, multiscale diffusion (rotational and translational) of a small fluorophore will be used to understand the role of non-specific binding, heterogeneity in microenvironmental viscosity in crowded solutions. Our experimental approach is a combination of fluorescence lifetime imaging microscopy, time-resolved anisotropy and fluorescence correlation spectroscopy. The broader impacts of these results will be discussed within the context of energy metabolism and biophysics in the crowded milieu of living cells.

Publisher

International Symposium on Molecular Spectroscopy

Type of Resource

text

Language

en

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http://hdl.handle.net/2142/55863

Copyright and License Information

Copyright 2014 by the authors. Licensed under a Creative Commons Attribution 4.0 International License. http://creativecommons.org/licenses/by/4.0/

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