Effects of stiffness gradient and hydrostatic pressure on cell migratory behaviors
Joaquin, Danielle
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https://hdl.handle.net/2142/49651
Description
Title
Effects of stiffness gradient and hydrostatic pressure on cell migratory behaviors
Author(s)
Joaquin, Danielle
Issue Date
2014-05-30T17:03:00Z
Director of Research (if dissertation) or Advisor (if thesis)
Hsia, K. Jimmy
Department of Study
Mechanical Sci & Engineering
Discipline
Mechanical Engineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
Mechanobiology
Tissue Engineering
Biological stiffness gradient
Cell migration
Cell motility
Cellular organization
Pressurized incubation chamber
Scratch Assay
Abstract
This thesis explores the effects of mechanical stimuli on cell patterning and behavior, which may influence our understanding of in vivo processes such as morphogenesis, wound healing, disease progression, tumor formation, and metastatic events. We begin by looking at the effects of a stiffness gradient in three-dimensional culture environment, which builds upon prior research on the effects of stiffness in a two-dimensional culture, but provides a platform for studying these outcomes that is more akin to the in vivo environment. Then we look at the effect of hydrostatic pressure using a non-contact pressure chamber that provides a uniform pressure environment while maintaining physiological conditions needed for cell growth. By seeking to better understand the migratory responses of cells to various mechanical stimuli, we gain tools and knowledge that can be used to aid in diagnoses and treatment of a variety of diseases.
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