University of Illinois Urbana-Champaign

Software Development for Realtime Spatial Light Interference Microscopy

Kandel, Mikhail Eugene

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https://hdl.handle.net/2142/47449

Description

Title
Software Development for Realtime Spatial Light Interference Microscopy
Author(s)
Kandel, Mikhail Eugene
Contributor(s)
Popescu, Gabriel
Issue Date
2013-05
Keyword(s)
  • quantitative phase imaging
  • live cell imagining
  • data acquisition
  • SLIM
Abstract
Quantitative phase imaging (QPI) is an emerging field biology aimed at studying weakly scattering and absorbing specimens. Spatial light interference microscopy is a white-light based common-path QPI method with sensitivity approaching that of force sensing methods. This setup is unique in that collation occurs digitally, converting the challenges faced by optical alignment, instability, and toxicity, into computational workload. By collating images with varied phase shifts using a liquid crystal phase modulator (LCPM), it is possible to create a topological map of a transparent specimen. However, due to the temporal acquisition of the frames to extract the map, the speed is limited due to the LCPM and the camera. We have incorporated a fast LCPM and a fast sCMOS camera to increase the speed such that the phase map retrieval will be possible in real time. To this end, my thesis centers on scaling, automating and synchronizing the image acquisition and analyzing process and also developing a user interface to facilitate ongoing and future studies.
Type of Resource
text
Language
en
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http://hdl.handle.net/2142/47449

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