RNA-SEQ driven analysis of seasonal gene expression variation in miscanthus �� giganteus

Barling, Adam R.

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https://hdl.handle.net/2142/46926 Copy

Description

Title

RNA-SEQ driven analysis of seasonal gene expression variation in miscanthus �� giganteus

Author(s)

Barling, Adam R.

Issue Date

2014-01-16T18:26:33Z

Director of Research (if dissertation) or Advisor (if thesis)

Moose, Stephen P.

Doctoral Committee Chair(s)

Moose, Stephen P.

Committee Member(s)

• Hudson, Matthew E.
• Brown, Patrick J.
• Bernacchi, Carl J.

Department of Study

Crop Sciences

Discipline

Crop Sciences

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

• Ribonucleic Acid Sequencing (RNA-Seq)
• Miscanthus
• Biofuel

Abstract

Miscanthus × giganteus is a C4 grass that has generated a large amount of interest as a potential biofuel crop due to its high level of biomass production, its perenniality, and its sterility. In this study, two separate M. × giganteus RNA-Seq datasets were generated to help explore the characteristics of M. × giganteus at the level of gene expression: a ten-tissue dataset suitable for examining genes with tissue-preferred expression, and a twenty-four sample dataset for examining the changes in gene expression that occur over the growing season. Aided by these datasets, aspects and potential mediators of M. × giganteus’ seasonal developmental cycle and changes in the utilization, storage, and long-distance mobilization and remobilization of the essential nutrient nitrogen have been studied. These RNA-Seq datasets have been verified with RT-qPCR and compared to amino acid and elemental concentration profiles; as a result, many seasonal changes in gene expression corresponding to the growth and development of M. × giganteus have been documented in order to better define the traits that make this crop such an outstanding biofuel candidate.

Graduation Semester

2013-12

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http://hdl.handle.net/2142/46926

Copyright and License Information

Copyright 2013 Adam Robert Barling

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