University of Illinois Urbana-Champaign

Incorporation and analysis of bilayer constituents in nanodiscs

Baker, Catherine

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Catherine_Baker.pdf

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https://hdl.handle.net/2142/46921

Description

Title
Incorporation and analysis of bilayer constituents in nanodiscs
Author(s)
Baker, Catherine
Issue Date
2014-01-16T18:26:24Z
Director of Research (if dissertation) or Advisor (if thesis)
Sligar, Stephen G.
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Date of Ingest
2014-01-16T18:26:24Z
Keyword(s)
  • Nanodiscs
  • Lipid exchange
  • Soluble membrane protein library (SMPL)
  • Lipoprotein
  • Membrane scaffold protein
  • Gadolinium
Abstract
Nanodiscs, engineered lipoparticles composed of a lipid bilayer and two surrounding amphipathic proteins, have been utilized to house and solubilize integral membrane proteins and other molecules with large hydrophobic groups which limit their aqueous solubility. In the experiments described herein, lipids from the Nanodiscs’ bilayer, gadolinium chelates and integral membrane proteins were analyzed for their incorporation and stability within the Nanodisc structure. Four techniques are described for quantification of the exchange of lipids between Nanodiscs over time, including: scintillation counting, imaging proximity assay, NMR and TLC MALDI-MS. Additionally, the extent and longevity of inclusion of the hydrophobic gadolinium-chelate “C3lipo” doped into the Nanodisc bilayer was analyzed and preliminary cell uptake studies were performed. Finally, the incorporation of proteins from rat liver and fetal bovine brain membranes into Nanodiscs to create soluble membrane protein libraries is discussed.
Graduation Semester
2013-12
Permalink
http://hdl.handle.net/2142/46921
Copyright and License Information
Copyright 2013 Catherine Baker

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