University of Illinois Urbana-Champaign

Photonic crystal enhanced microscopy for the characterization of cell attachment

Lidstone, Erich

Content Files
Erich_Lidstone.pdf

Permalink

https://hdl.handle.net/2142/44771

Description

Title
Photonic crystal enhanced microscopy for the characterization of cell attachment
Author(s)
Lidstone, Erich
Issue Date
2013-05-28T19:19:15Z
Director of Research (if dissertation) or Advisor (if thesis)
Cunningham, Brian T.
Doctoral Committee Chair(s)
Cunningham, Brian T.
Committee Member(s)
  • Schook, Lawrence B.
  • Popescu, Gabriel
  • Underhill, Gregory
Department of Study
Bioengineering
Discipline
Bioengineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Date of Ingest
2013-05-28T19:19:15Z
Keyword(s)
  • photonic
  • photonic crystal
  • biosensor
  • label-free
  • optical
  • label-free cell attachment
  • cell adhesion
  • attachment density
  • guided mode resonance filter
Abstract
As basic science research has increased in both complexity and scale, so too has the importance of cost-effective and generalizable techniques for the interrogation of cells and tissues. Over the past few decades, several technologies have been leveraged to meet this demand, recently including photonic crystal (PC) biosensors. This work describes the application of PC biosensors to cell microscopy, resulting in a label-free imaging technique capable of measuring cell attachment and attachment modulation. The approach uses a photonic crystal optical resonator surface incorporated into conventional microplate wells and a microscope-based detection instrument that measures shifts in the resonant coupling conditions caused by localized changes in dielectric permittivity at the cell-sensor interface. Four model systems are demonstrated for studying cancer cells, primary cardiac muscle cells, and stem cells. Each experiment yielded information regarding cell attachment density without the use of potentially cytotoxic labels, enabling study of processes including growth, development, differentiation, and death in the same cells for periods lasting several days. After demonstrating this technology in several systems typical of cell biology problems, PC biosensors were applied toward the investigation of tumor immunity. In particular, it has remained difficult for immunologists to study how tumor cells interact with components of the innate immune system, especially in the context of specific apoptotic recognition. The final chapter details the use of PC biosensors in detecting these types of interactions between two distinct cell types, the first reported use of label-free biosensors to detect such cellular changes.
Graduation Semester
2013-05
Permalink
http://hdl.handle.net/2142/44771
Copyright and License Information
Copyright 2013 Erich Lidstone

Owning Collections

Dissertations and Theses - Bioengineering
Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois