University of Illinois Urbana-Champaign

Mid infrared spectroscopic imaging and tomography

Reddy, Rohith

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Rohith_Reddy.pdf

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https://hdl.handle.net/2142/44759

Description

Title
Mid infrared spectroscopic imaging and tomography
Author(s)
Reddy, Rohith
Issue Date
2013-05-28T19:18:36Z
Director of Research (if dissertation) or Advisor (if thesis)
Bhargava, Rohit
Doctoral Committee Chair(s)
Bhargava, Rohit
Committee Member(s)
  • Carney, Paul S.
  • Do, Minh N.
  • Ahuja, Narendra
Department of Study
Bioengineering
Discipline
Bioengineering
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Date of Ingest
2013-05-28T19:18:36Z
Keyword(s)
  • Fourier Transform Infrared (FT-IR) spectroscopic Imaging
  • Mid-Infrared Spectroscopic Tomography
  • Spectroscopic Imaging
  • Spectroscopic Tomography
  • mid-Infrared (Mid-IR)
  • Spectroscopy
  • Imaging
Abstract
Fourier Transform Infrared (FT-IR) Spectroscopic Imaging is an optical technique that simultaneously provides morphological (two dimensional) and mid-infrared spectral (one dimensional) information for a specimen under observation. In this dissertation, we develop a new instrument that extends the capabilities of FT-IR imaging to provide three dimensional morphological information along with mid-infrared spectral information on the microscopic length scale. We first demonstrate that spectroscopic information from the mid-infrared can be used to perform histologic recognition and cancer detection in prostate, breast and skin cancer tissues. These studies utilize FT-IR imaging data of thin tissue slices from several hundred patients. The segmentation algorithms and instrumentation used are described in detail. We perform a rigorous analysis of instrumentation and data so as to understand the limits and limitations of current state-of-the-art instrumentation. We then propose a new instrument that adds a new dimension of information to current instruments and establish the new state-of-the-art in mid-infrared imaging. The theoretical basis of such instrumentation is presented in detail and techniques for visualization of four dimensional data are outlined.
Graduation Semester
2013-05
Permalink
http://hdl.handle.net/2142/44759
Copyright and License Information
Copyright 2013 Rohith Reddy

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