High resolution optical DNA mapping

Baday, Murat

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https://hdl.handle.net/2142/44261 Copy

Description

Title

High resolution optical DNA mapping

Author(s)

Baday, Murat

Issue Date

2013-05-24T22:05:51Z

Director of Research (if dissertation) or Advisor (if thesis)

Selvin, Paul R.

Doctoral Committee Chair(s)

Schroeder, Charles M.

Committee Member(s)

• Selvin, Paul R.
• Nayfeh, Munir H.
• Myong, Su-A
• Kuhlman, Thomas E.

Department of Study

School of Molecular & Cell Bio

Discipline

Biophysics & Computnl Biology

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

• Super-resolution
• optical DNA mapping
• genomic variations
• copy-number variations
• Single molecule-High Resolution Imaging with Photobleaching (SHRImP)
• High-Resolution Co-localization (SHREC)
• Deoxyribonucleic Acid (DNA)

Abstract

Many types of diseases including cancer and autism are associated with copy-number variations in the genome. Most of these variations could not be identified with existing sequencing and optical DNA mapping methods. We have developed Multi-color Super-resolution technique, with potential for high throughput and low cost, which can allow us to recognize more of these variations. Our technique has made 10–fold improvement in the resolution of optical DNA mapping. Using a 180 kb BAC clone as a model system, we resolved dense patterns from 108 fluorescent labels of two different colors representing two different sequence-motifs. Overall, a detailed DNA map with 100 bp resolution was achieved, which has the potential to reveal detailed information about genetic variance and to facilitate medical diagnosis of genetic disease.

Graduation Semester

2013-05

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http://hdl.handle.net/2142/44261

Copyright and License Information

Copyright 2013 Murat Baday

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