University of Illinois Urbana-Champaign

Phase derivative microscopy of biological cells and tissues

Kim, Tae Woo

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Tae Woo_Kim.pdf

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https://hdl.handle.net/2142/44103

Description

Title
Phase derivative microscopy of biological cells and tissues
Author(s)
Kim, Tae Woo
Issue Date
2013-05-24T21:50:48Z
Director of Research (if dissertation) or Advisor (if thesis)
Popescu, Gabriel
Department of Study
Electrical & Computer Eng
Discipline
Electrical & Computer Engr
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Date of Ingest
2013-05-24T21:50:48Z
Keyword(s)
  • Microscopy
  • Cell analysis
  • Coherence Imaging
  • Optical pathology
  • Tissue characterization
Abstract
Phase derivative microscopy (PDM), including Laplace field microscopy (LFM) and gradient field microscopy (GFM), generates high contrast images of transparent specimens. These methods utilize spatial light modulation at the Fourier plane of a bright field microscope to optically obtain the derivatives of the phase and increase the contrast of the final image. The controllable spatial modulation pattern allows obtaining both the first-order and the second-order derivative of the phase. Compared to differential interference contrast (DIC) microscopy, which is a traditional method of imaging the derivative of the phase, PDM shows higher stability because there are no mechanically moving parts involved in the operation. More importantly, unlike DIC, PDM does not use polarizing optics and, thus, it is applicable to birefringent samples. Furthermore, because it is a single-shot technique, PDM allows acquisition speed as fast as the detector allows, and therefore, it is suitable for studying live biological samples. This thesis introduces the principle of PDM, the setup, and also applications of PDM, including high contrast imaging, live cell dynamics study, and unstained tissue biopsies.
Graduation Semester
2013-05
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http://hdl.handle.net/2142/44103
Copyright and License Information
Copyright 2013 Tae Woo Kim

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