Fast Fluorescence Lifetime Imaging Using a Full-Field Homodyne System with Applications in Biology
Redford, Glen Ivan
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https://hdl.handle.net/2142/35217
Description
Title
Fast Fluorescence Lifetime Imaging Using a Full-Field Homodyne System with Applications in Biology
Author(s)
Redford, Glen Ivan
Issue Date
2005-12
Doctoral Committee Chair(s)
Clegg, Robert M.
Department of Study
Physics
Discipline
Physics
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Fluorescence Lifetimes
Imaging
Fluorescence signals
Chebyshev polynomials
Full-field homodyne
Language
en
Abstract
A fast, full-field fluorescence lifetime imaging system based on the homodyne technique was built and used for several applications. Also contained herein is the basic theory and background for fluorescence lifetime imaging, as well as data analysis of lifetime distributions. The fast, easy-to-use lifetime imager has proven useful for many applications and essential for several. Examples of applications where such an instrument is needed (and where a conventional system is inadequate) include: any system with large photo-bleaching effects, systems where the intensity changes on the timescale of the measurement, and situations that require real-time display of lifetime images. Measuring proto-porphyrin IX in cells is an example of a system where photobleaching makes it difficult to measure. Measuring the lifetime changes in dark adapted plant leaves upon exposure to light is an example of a system with complicated intensity changes. Real-time display of lifetime images is required for clinical work. This paper includes details of the hardware and software for the instrument as well as data taken with the instrument.
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