Biophysical Applications of Single Molecule Fluorescence Localization
Gordon, Matthew Paul
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Permalink
https://hdl.handle.net/2142/34737
Description
Title
Biophysical Applications of Single Molecule Fluorescence Localization
Author(s)
Gordon, Matthew Paul
Issue Date
2006-10
Doctoral Committee Chair(s)
Selvin, Paul R.
Department of Study
Physics
Discipline
Physics
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Fluorescence Spectroscopy
Genetic Haplotypes
Photobleaching
Language
en
Abstract
Fluorescence single molecule localization has become a powerful tool for investigating problems in biological physics. However, there are numerous limitations on the types of measurements that can be made with typical instrument designs based on objective-type TIR and wide field CCD imaging. We present several novel applications of single molecule localization to both proteomics and genomics, including a method for improving the resolving capacity by more than an order of magnitude (Single Molecule High Resolution Imaging with Photo-bleaching, or SHRImP), a method for extending wide field single-molecule localization to three dimensions (Three Dimensional Imaging with One Nanometer Accuracy, or 3IONA), and applications of these techniques to problems in genomics and molecular motors.
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