A microfluidic platform for hematopoietic stem cell separation

Zhang, Jieqian

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https://hdl.handle.net/2142/32058 Copy

Description

Title

A microfluidic platform for hematopoietic stem cell separation

Author(s)

Zhang, Jieqian

Issue Date

2012-06-27T21:30:54Z

Director of Research (if dissertation) or Advisor (if thesis)

Kenis, Paul J.A.

Doctoral Committee Chair(s)

Leckband, Deborah E.

Committee Member(s)

• Kenis, Paul J.A.
• Gaskins, H. Rex
• Bailey, Ryan C.

Department of Study

Chemistry

Discipline

Chemistry

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

• Microfluidics
• cell enrichment
• hematopoietic stem cell

Abstract

Microfluidics manipulates, processes, and controls small volumes of fluids in channels or compartments with cross-sectional dimensions on the order of 10-100 µm. By integrating functional components such as channels, actuators, and sensors, and by leveraging characteristic physical phenomena occurring at the microscale, microfluidics brings microfabrication, chemistry and physics together to develop new experimental paradigm for broad applications, including the study of biological problems. Indeed, microfluidics operates at a scale that is particularly suitable for biological sample analysis, which provides great opportunities for technology innovation in bioanalytical assays and biomedical applications such as point-of-care devices. Challenges due to the complexity of the biological system can be overcome by introducing new engineering elements and performing systematic device optimization. In this work, a microfluidic immunoaffinity based silicon micropost device for hematopoietic stem cell enrichment is discussed, which demonstrates how innovations in microfluidics can revolutionize current clinical therapies. Appropriate surface chemistry for robust immobilization of proteins on the surface of different materials and topography was developed. Silicon chips with three-dimensional features, arrays of microposts in a wide range of arrangements, were designed. The fluid dynamics in the various micropost arrays was modeled and optimized to meet specific needs of cell enrichment for clinical application. The optimized three-dimensional micropost devices achieve hematopoietic stem cell enrichment at a level that outperforms all current technologies and successfully meets the requirements for clinical transplantation applications.

Graduation Semester

2012-05

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http://hdl.handle.net/2142/32058

Copyright and License Information

Copyright 2012 Jieqian Zhang

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