University of Illinois Urbana-Champaign

Near infrared transient absorption spectra of photolyzed myoglobin at low temperatures

Bowne, Samuel Franklin

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https://hdl.handle.net/2142/25311

Description

Title
Near infrared transient absorption spectra of photolyzed myoglobin at low temperatures
Author(s)
Bowne, Samuel Franklin
Issue Date
1984
Doctoral Committee Chair(s)
Frauenfelder, Hans
Department of Study
Physics
Discipline
Physics
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • transient absorption spectrum
  • photolyzed carboxymyoglobin
  • band shifts
Language
en
Abstract
"We have measured the transient absorption spectrum of photolyzed sperm whale carboxymyoglobin as a function of temperature and time after photolysis. Our measurements extend from 60K to 220K and from three microseconds to thirty seconds. We see an absorption band near 758 nm which is known as band VIII. Three microseconds after photolysis at 60K, the center of band VIII is 766 nm, red-shifted from the peak seen in deoxy myoglobin at this temperature. Band VIII shifts with time towards the deoxy value with a nonexponential time course. We conclude that this shift is caused by unrelaxed degrees of freedom in the protein at these temperatures. We present a model in which each protein has several ""blocking residues"" which relax following photolysis by surmounting enthalpy barriers which range from ten to fifty kJ/mol. These motions do not affect the rate of ligand rebinding."
Type of Resource
text
Permalink
http://hdl.handle.net/2142/25311
Copyright and License Information
1984 Samuel Franklin Bowne

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Dissertations and Theses - Physics
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Graduate Theses and Dissertations at Illinois