Enzymatic diversity in lipoic acid modification of proteins
Christensen, Quin H.
Loading…
Permalink
https://hdl.handle.net/2142/24498
Description
Title
Enzymatic diversity in lipoic acid modification of proteins
Author(s)
Christensen, Quin H.
Issue Date
2011-05-25T14:22:47Z
Director of Research (if dissertation) or Advisor (if thesis)
Cronan, John E.
Doctoral Committee Chair(s)
Cronan, John E.
Committee Member(s)
Imlay, James A.
Metcalf, William W.
Olsen, Gary J.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
lipoic acid
amidotransfer
Bacillus
Thermoplasma
Abstract
Escherichia coli is one of a few organisms with a well characterized and complete metabolic model for lipoic acid metabolism. In this thesis I make discoveries through analysis of the diversity of lipoic acid metabolic genes in other organisms. By examining the distribution and phylogeny of the lipoate ligase protein family, I and others have found gaps and inconsistencies in our knowledge of lipoic acid metabolic pathways. I examine select proteins through heterologous expression in E. coli, allowing complementation studies and biochemical analysis. This enables prediction of lipoic acid metabolism in various organisms and reveals novel pathways and enzymology.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
