University of Illinois Urbana-Champaign

A stable genomic source of P element transposase in Drosophila melanogaster

Robertson, Hugh M.; Preston, Christine R.; Phillis, Randall W.; Johnson-Schlitz, Dena M.; Benz, Wendy K.; Engels, William R.

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https://hdl.handle.net/2142/218

Description

Title
A stable genomic source of P element transposase in Drosophila melanogaster
Author(s)
  • Robertson, Hugh M.
  • Preston, Christine R.
  • Phillis, Randall W.
  • Johnson-Schlitz, Dena M.
  • Benz, Wendy K.
  • Engels, William R.
Issue Date
1988-03
Keyword(s)
  • Drosophila melanogaster
  • Genetics
Abstract
A single P element insert in Drosophila melanogoster, called P[ry+A2-3](99B), is described that causes mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for “helper” plasmids in P element mediated transformation, and can be used to move transformed elements around the genome.
Publisher
Genetics Society of America
Type of Resource
text
Language
en
Permalink
http://hdl.handle.net/2142/218
Copyright and License Information
Copyright owned by Genetics Society of America

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