The morphology and function of Peyer's patches in the dog
HogenEsch, Harm
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Permalink
https://hdl.handle.net/2142/23106
Description
Title
The morphology and function of Peyer's patches in the dog
Author(s)
HogenEsch, Harm
Issue Date
1989
Doctoral Committee Chair(s)
Felsburg, P.J.
Department of Study
Pathobiology
Discipline
Pathobiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Veterinary Science
Health Sciences, Immunology
Language
eng
Abstract
Canine Peyer's patches (PPs) were characterized by immunohistology, alkaline phosphatase histochemistry and electron microscopy. Duodenal PPs were very similar to jejunal PPs, except for the consistent presence of intrafollicular invaginations of the dome epithelium. The single ileal PP had small domes and sparsely populated interfollicular areas. Whereas the dome epithelium of proximal PPs consisted of alkaline phosphates positive enterocytes and scattered M cells with low alkaline phosphatase activity, ileal dome epithelia predominantly consisted of M cells and few enterocytes. The domes of PPs contained numerous IgG plasma cells and a few or moderate number of IgA plasma cells.
The development of PPs in the dog began before birth, but germinal centers and significant mitotic activity were not observed until after 1 week post partum. The ileal PP reached a large size in 4-6 months old dogs and rapidly involuted afterwards.
Cell suspensions from proximal and ileal PPs contained approximately 50% B cells, but ileal PPs contained more mIgM$+$ cells and fewer mIgA$+$ cells. Fewer T cells were present in the ileal PP. Cells from proximal PPs secreted more IgA than cells from other lymphoid tissues in vitro. The ileal PP secreted very little IgG and IgA, but more IgM than proximal PPs.
IgA, but not IgG and IgM, secretion by pokeweed mitogen-stimulated cells from proximal PPs was further enhanced by addition of concanvalin A (ConA) to the cultures, suggesting the presence of isotype-specific T cells. This effect of ConA was not observed in other lymphoid tissues. ConA-activated T cells were expanded in the presence of interleukin-2 and cloned by limiting dilution. One clone induced a large secretion of IgA by PWM stimulated B cells, isolated by panning from peripheral blood or peripheral lymph nodes.
It was concluded that proximal PPs of dogs are important in IgA production; that PP T cells can increase IgA production; and that the ileal PP is morphologically and functionally different from proximal PPs and may be a site of B cell amplification in the dog.
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