University of Illinois Urbana-Champaign

Characterization and analysis of genes encoding Escherichia coli acetyl-CoA carboxylase

Li, Shyr-Jiann

This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.

Permalink

https://hdl.handle.net/2142/23083

Description

Title
Characterization and analysis of genes encoding Escherichia coli acetyl-CoA carboxylase
Author(s)
Li, Shyr-Jiann
Issue Date
1992
Doctoral Committee Chair(s)
Cronan, John E.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • Biology, Molecular
  • Biology, Microbiology
Language
eng
Abstract
  • Acetyl-CoA carboxylase catalyzes the first committed step of fatty acid biosynthesis, the synthesis of malonyl-CoA. In Escherichia coli the enzyme is a complex of four subunits that catalyzes two distinct half-reactions.
  • I have cloned and determined the nucleotide sequences of the genes encoding biotin carboxylase, BCCP (map at min 72), and carboxyltransferase subunits (The $\alpha$ subunit map at min 4.3 and the $\beta$ subunit map at min 50). I have also defined the sequence requirement for biotination of the BCCP subunit by protein fusion analyses. Peptide mapping of the purified carboxyltransferase indicates that this enzyme component is a complex of two nonidentical subunits. In addition, I have identified putative functional domains within the deduced amino acid sequences. The identified domains include sequences that involves in ATP, bicarbonate binding (biotin carboxylase), in biotination (BCCP), and in CoA binding (carboxyltransferase).
  • To investigate the regulation of acetyl-CoA carboxylase genes, I monitored the transcription levels of these genes under a variety of growth conditions. A direct correlation was found between the levels of acc genes transcripts and the rate of cellular growth.
Type of Resource
text
Permalink
http://hdl.handle.net/2142/23083
Copyright and License Information
Copyright 1992 Li, Shyr-Jiann

Owning Collections

Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Microbiology