High-resolution nuclear magnetic resonance studies of lipids and model membranes
Shan, Xi
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Permalink
https://hdl.handle.net/2142/22662
Description
Title
High-resolution nuclear magnetic resonance studies of lipids and model membranes
Author(s)
Shan, Xi
Issue Date
1990
Doctoral Committee Chair(s)
Oldfield, Eric
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Physical
Biophysics, General
Language
eng
Abstract
"This thesis is devoted to the new applications of high resolution $\sp1$H and $\sp{13}$C ""magic angle sample spinning"" (MASS) nuclear magnetic resonance (NMR) techniques to the study of model and biological membranes. Since the resolution of $\sp1$H and $\sp{13}$C MASS NMR spectra from multilamella and hexagonal-II phase is the same or even better than that obtained with sonicated liquid-crystalline system, the dynamics of membranes can be studied through measuring linewidth, T$\sb1$, T$\sb{1\rho}$, T$\sb2$ relaxation rates for numerous, resolved, single carbon, or individual proton sites by MASS NMR techniques. Chapter II provides a summary of the theoretical background of MASS NMR of model membranes and spin-lattice relaxations of bilayer system. Chapter III discusses the application of high resolution $\sp1$H MASS NMR to the study of a variety of lipids. The chemical shift assignments, order parameters, and relaxation times (T$\sb1$, T$\sb{1\rho}$, T$\sb2$) can be determined for these lipids. Chapter IV reports the study of a typical model system for lipid-protein interaction in membrane, gramicidin-phospholipid, by using $\sp1$H MASS NMR techniques. The dynamic picture of phospholipid-polypeptide interaction is obtained. Chapter V discusses the application of high resolution $\sp1$H and $\sp{13}$C MASS NMR spectroscopy to the study of thylakoid lipids, monogalactosyldiacyglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and sulphoquinovosyldiacylglycerol (SQDG). The chemical shift assignments and the dynamic picture is derived for these thylakoid lipids. Results on studying a variety of phospholipids and sphingomyelin by using $\sp{13}$C MASS NMR spectroscopy are presented in Chapter VI."
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