Molecular response of soybean (Glycine max (L.) Merr) to Phialophora gregata
Monte-Neshich, Damares De Castro
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https://hdl.handle.net/2142/22330
Description
Title
Molecular response of soybean (Glycine max (L.) Merr) to Phialophora gregata
Author(s)
Monte-Neshich, Damares De Castro
Issue Date
1989
Doctoral Committee Chair(s)
Hepburn, Angus G.
Department of Study
Agronomy
Discipline
Agronomy
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Agriculture, Agronomy
Biology, Molecular
Agriculture, Plant Pathology
Language
eng
Abstract
The soybean disease Brown Stem Rot is caused by infection by the fungus Phialophora gregata. Several cultivars of soybean (Glycine max (L.) Merr.) show levels of resistance to attack by the fungus. In order to determine the mechanism of this resistance, the response of whole plants and soybean cells in culture was studied at the biochemical and molecular level. Soybean cultured cells were treated with culture filtrate of the fungus and the proteins synthesized by the plant cells were extracted at times after treatment. Both susceptible and resistant soybean cells responded to exposure to the filtrate by synthesizing several novel proteins. A limited number of these appeared to be specific to the resistant cells treated with the filtrate and could be considered as suitable candidates for proteins that are directly involved in the resistant response. cDna libraries were made from soybean cells treated with the filtrate and screened by hybridization to mRNA isolated from resistant control and treated tissues. This allowed the isolation of 10 clones whose expression increased significantly in resistant cells following treatment. These clones were purified and their expression was determined in whole plant tissues (leaves and stem) from susceptible and resistant cultivars after infection with Phialophora gregata and at various stages in the progression of the disease as estimated from the kinetics of symptom development. The clones showed different induced responses in the two cultivar type (resistant or susceptible). One clone (pG5-2) showed a specific induction in the leaves or stem only when a resistant response occurred, either in the resistant cultivar infected with the non-pathogenic fungus.
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