Use of estradiol-BSA conjugates to characterize membrane estrogen binding sites in brain cells: Biochemical and physiological studies
Zheng, Jianbiao
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Permalink
https://hdl.handle.net/2142/21798
Description
Title
Use of estradiol-BSA conjugates to characterize membrane estrogen binding sites in brain cells: Biochemical and physiological studies
Author(s)
Zheng, Jianbiao
Issue Date
1996
Doctoral Committee Chair(s)
Ramirez, Victor D.
Department of Study
Neuroscience
Discipline
Neuroscience
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Biology, Neuroscience
Biology, Animal Physiology
Language
eng
Abstract
Rapid nongenomic actions of estrogen probably involve one or several membrane estrogen receptors/binding proteins, which are different from classical estrogen receptor (ER). Specific membrane binding sites for estrogen (mEBS) in female rat brain were demonstrated by a novel and sensitive radioligand binding assay using 17$\beta$-estradiol covalently linked with ($\sp{125}$I) -labeled bovine serum albumin (17$\beta$-E-6- ($\sp{125}$I) BSA conjugate), as a ligand. These membrane binding sites (dissociation constants of 3-34 nM and total binding sites of 3-40 pmol/mg protein) in crude synaptosomal fractions (P2) from hypothalamus, olfactory bulb, and cerebellum show distinct binding specificity from classical ER. To establish if the mEBS identified by 17$\beta$-E-6- ($\sp{125}$I) BSA represent putative membrane estrogen binding proteins, a ligand blotting technique was used. Three major binding proteins with molecular weights of about 23, 28, and 32 kDa, and three additional proteins (18, 40, and 130 kDa), were specifically labeled. The 23 and 40 kDa proteins were concentrated in mitochondrial fractions (mP2) whereas 18, 28, and 32 kDa proteins were enriched in microsomal fractions (P3). One of these membrane estrogen binding proteins (23 kDa) was purified from digitonin-solubilized P2 fractions by affinity columns coupled with 17$\beta$-E-6-BSA and was identified as the oligomycin-sensitivity conferring protein (OSCP), a subunit of the F$\sb0$F$\sb1$ ATP-synthase/ATPase required for the coupling of a proton gradient across the F$\sb0$ sector of the mitochondrial membrane to ATP synthesis in the F$\sb1$ sector. OSCP, therefore, represents an estrogen target site in addition to ER in the cells and may play an important role in estrogen-induced changes in cell energy metabolism. The unlabeled ligand, 17$\beta$-E-6-BSA (1-100 nM), was biologically active in rapidly facilitating the release of dopamine from corpus striatum, an important estrogen target site that lacks classical ER. This rapid action of estrogen is consistent with the presence of mEBS in the plasma membranes of these cells and future work is ongoing to purify this putative mEBS and/or clone its cDNA.
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