University of Illinois Urbana-Champaign

Enzyme-linked immunosorbent assay for herbicide detection

Koppatschek, Fritz K.

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https://hdl.handle.net/2142/21758

Description

Title
Enzyme-linked immunosorbent assay for herbicide detection
Author(s)
Koppatschek, Fritz K.
Issue Date
1989
Doctoral Committee Chair(s)
Liebl, Rex A.
Department of Study
Crop Sciences
Discipline
Crop Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • Agriculture, Agronomy
  • Chemistry, Agricultural
Language
eng
Abstract
  • The purpose of this research was to develop polyclonal antibodies to the soybean herbicides clomazone (2- ((2 chlorophenyl)methyl) -4,4-dimethyl-3-isoxazolidinone), imazaquin (2- (4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl) -3-quinolinecarboxylic acid), and metribuzin (4-amino-6-(1,1-dimethylethyl)-3-(methylthio)-1,2,4-triazin-5(4H)-one). The herbicide-specific antibodies were to be used in an enzyme linked immunosorbent assay (ELISA) for herbicide detection in soil.
  • Antibodies, specific to clomazone, were produced in New Zealand white rabbits to a BSA-clomazone conjugate. Attempts at producing antibodies specific to imazaquin, via BSA-imazaquin and BSA-hexanoic acid-imazaquin conjugates, were not successful. Antibodies specific to metribuzin, via a porcine gamma globulin-metribuzin conjugate, were not successfully produced.
  • The antibodies to the BSA-clomazone conjugate were sensitive to clomazone having an I$\sb{50}$ of 12 ng/mL. The antibodies were also specific to clomazone and demonstrated no cross reactivity to the soybean herbicides metribuzin, metolachlor (2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide) or trifluralin (2,6-dinitro-N,N-dipropyl-4-(trifluromethyl)benzenamine).
  • Soil extracts, from several different soil types, did not interfere with detection of clomazone in this ELISA. Soil extraction procedures could also be simplified without reducing the sensitivity of the assay.
  • The ELISA system compared favorably with conventional clomazone detection systems of wheat bioassay and gas chromatography. The low level of detection for both gas chromatography and ELISA was from a 10 ppb acetonitrile extract of clomazone treated soil.
  • Clomazone-treated soil was extracted with phosphate buffer and clomazone was quantified with ELISA. Detection of clomazone in the phosphate buffer extracts was influenced by soil type, with greater detection in low organic matter soil. This technique offers a novel method for quantifying a portion of the biologically available herbicide.
  • The ELISA system proved to be a fast, accurate and sensitive method of clomazone detection.
Type of Resource
text
Permalink
http://hdl.handle.net/2142/21758
Copyright and License Information
Copyright 1989 Koppatschek, Fritz K.

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Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Crop Sciences