Effects of dietary heated fats and cyclic fatty acid monomers on rat liver enzyme activity
Lamboni, Courdjo
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Permalink
https://hdl.handle.net/2142/21580
Description
Title
Effects of dietary heated fats and cyclic fatty acid monomers on rat liver enzyme activity
Author(s)
Lamboni, Courdjo
Issue Date
1993
Doctoral Committee Chair(s)
Perkins, Edward G.
Department of Study
Nutritional Sciences
Discipline
Nutritional Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Chemistry, Biochemistry
Health Sciences, Nutrition
Language
eng
Abstract
The objective of this research was to evaluate the effects of dietary heated fats from a commercial deep-fat frying operation and of cyclic fatty acids (CFA) on rat liver enzyme activity. The fats were partially hydrogenated soybean oil (PHSBO) used 4 days (4-MH) and 7 days (7-DH) for frying foodstuffs. Balanced diets containing these used fats were fed to rats for 10 weeks. In a second experiment, rats were pair-fed diets containing graded doses of 7-DH. In a third, 0.15% CFA diet was fed to animals. In a fourth, rats were pair-fed diet containing the 7-DH treated (T-7DH) with magnesium silicate which removes some polymers from the used oil. All four sets of experiments were conducted with control rats fed non-heated PHSBO (NH) diet. All diets were isocaloric with 15% fat.
Animals fed 7-DH diet either ad libitum or by pair-feeding, showed significant increased contents of cytochromes P$\sb{450}$ and b$\sb5$ as well as increased activity of (EC 1.6.2.4) NADPH-cytochrome P$\sb{450}$ reductase when compared to controls. The 4-MH diet group showed the same trends for cytochromes contents and reductase activity in comparison to controls. Similar results were obtained with CFA diet. The activities of (EC 2.3.1.21) carnitine palmitoyltransferase-I; (EC 1.1.1.42) isocitrate dehydrogenase; were significantly decreased in each case either ad libitum or by pair-feeding in comparison to controls. Significantly depressed activity of (EC 1.1.1.49) glucose 6-phosphate dehydrogenase was also noticed for these animals when compared to NH. Similar results were recorded when fed CFA diet to rats. In addition, liver and microsomal protein were significantly increased when the used oils were fed to rats either ad libitum or by pair-feeding in comparison to NH. Enlarged and fatty livers were noted. Similar results were obtained when fed CFA diet. Liver glycogen was significantly decreased when fed the used oils or CFA diets in comparison to controls. However, when animals were fed T-7DH diet, the activities of the enzymes were significantly improved.
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