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https://hdl.handle.net/2142/21233
Description
Title
Studies on the cone pigment, iodopsin
Author(s)
Chen, Jian-Guo
Issue Date
1989
Doctoral Committee Chair(s)
Ebrey, Thomas G.
Department of Study
Biophysics and Computational Biology
Discipline
Biophysics
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biophysics, Medical
Language
eng
Abstract
I. Three kinds of monoclonal antibodies have been found against iodopsin and other cone pigments in chicken retina: (1) specifically against iodopsin (cell line of 7D3); (2) against iodopsin and another cone pigment (cell lines of 2D4, 4G2, and 5E3); and (3) against iodopsin and other three cone pigments (cell line of 2C3). One dimensional immunoblottings show that apparent molecular weight of iodopsin is about 32 kD and apparent molecular weights of other three cone pigments are about 29 kD, 31 kD, and 35 kD. Two dimensional immunoblottings show that iodopsin (and probably for the other cone pigments too) has a high isoelectric point value.
II. A series of synthetic 9-cis-, 11-cis-dehydro- and dihydro-retinals has been used to study the wavelength regulation in iodopsin by artificial pigments' regeneration. A model of the wavelength regulation has been proposed that: (1) a weaker nitrogen/counter-ion interaction is considered in the Schiff base region of the 11-cis retinal; (2) a second negative charge is located near the $\beta$-ionone ring of the retinal; and (3) a planar-s-trans ring/chain conformation is hypothesized.
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