University of Illinois Urbana-Champaign

Molecular genetic analysis of puf andpuc operon expression in Rhodobacter sphaeroides 2.4.1

Lee, Jeong Kug

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Description

Title
Molecular genetic analysis of puf andpuc operon expression in Rhodobacter sphaeroides 2.4.1
Author(s)
Lee, Jeong Kug
Issue Date
1992
Doctoral Committee Chair(s)
Gardner, Jeffrey F.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • Biology, Molecular
  • Biology, Microbiology
Language
eng
Abstract
  • The deletion of the puf intercistronic terminator resulted in both the loss of the smallest 0.5-kilobase (kb) puf transcript in addition to an approximate 10-fold increase in transcriptional read-through of the mutated region to more distal pufL gene, suggesting that the proximal intercistronic stem-loop functions as a transcription terminator.
  • The puc operon encodes 2.3- and 0.5-kb transcripts. The 2.3-kb transcript extends from the same 5$\sp\prime$ end (117 nucleotides from the start of pucB) as that of the 0.5-kb transcript to approximately 1.8 kb downstream of the pucBA genes, and encodes gene product(s) involved in post-translational expression of the pucBA gene products involved in the formation of B800-850 complex.
  • A 629-base pair DNA region upstream of the 5$\sp\prime$-ends of the two puc-specific transcripts encompasses two functionally separable cis-acting domains: the upstream regulatory region (URS, $-$629 to $-$150) responsible for oxygen and light control of puc operon transcription, and the more proximal downstream regulatory region (DRS, $-$150 to $-$1) containing putative IHF/FNR-binding sites ($-$129 $-$105), putative promoter(s) ($-$92 and $-$57), and operator(s) ($-$52 $-$35 and $-$27 $-$10) involved in oxygen control of puc operon transcription. In addition, data reveal a direct interaction between the URS ($-$629 $-$150) and the DRS ($-$150 $-$1). A trans-acting factor involved in O$\sb2$ control of puc operon transcription acted upon the puc URS, while a second trans-acting factor(s) acted upon both the puc URS and DRS. The former trans-acting factor was genetically mapped to a 2.2-kb DNA fragment located within the carotenoid gene cluster, while the genetic map of the second trans-acting factor was localized to a 7.0-kb DNA fragment containing the puhA gene and its flanking DNA (6.3 kb).
Type of Resource
text
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http://hdl.handle.net/2142/21113
Copyright and License Information
Copyright 1992 Lee, Jeong Kug

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Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Microbiology