The role of plant hormones, carbohydrates and indolebutyric acid in root and shoot development of two transplanted woody species
Cappiello, Paul Emmanuel
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https://hdl.handle.net/2142/20915
Description
Title
The role of plant hormones, carbohydrates and indolebutyric acid in root and shoot development of two transplanted woody species
Author(s)
Cappiello, Paul Emmanuel
Issue Date
1989
Doctoral Committee Chair(s)
Kling, Gary J.
Department of Study
Crop Sciences
Discipline
Crop Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Plant Physiology
Language
eng
Abstract
Field and laboratory experiments were conducted to examine the roles of endogenous and applied plant hormones in the control of root and shoot development of woody plants. An experiment was conducted to determine the effectiveness of IBA root sprays applied to seedling red oaks up to two weeks prior to planting. Bare root Quercus rubra seedlings were sprayed with 0, 3000, 6000 and 9000 ppm of IBA at 0, 3, 7 and 14 days prior to planting in containers or in the field. After the second year of field growth, plants treated with 9000 ppm of IBA on the planting date and 3 and 14 days prior to planting, produced 49.9, 122.2 and 110.4% increases in shoot growth over plants that received no IBA. Plants that received 6000 ppm of IBA showed the greatest amount of shoot growth after 3 years regardless of treatment time. The results indicate that root regeneration and shoot growth of red oak can be increased with IBA root sprays applied up to two weeks prior to planting.
Solid phase extraction and ion exchange high pressure liquid chromatography (HPLC) techniques were coupled in the development of an analytical procedure for quantitation of zeatin and zeatin riboside. A previous study described a preparative HPLC system for initial purification of abscisic acid (ABA), indoleacetic acid (IAA), zeatin and zeatin riboside. Final quantitative procedures for zeatin and zeatin riboside analysis have been reported previously. These coupled systems will make possible, more complete studies of the interactive nature of plant hormones than were possible previously.
The above system was used in an experiment which monitored levels of ABA, IAA, zeatin, zeatin riboside glucose, fructose, sucrose and starch in roots and shoot apices of red twig dogwood (Cornus sericea) plants during simulated cold storage, through bud break and into active vegetative growth.
Bud break was associated with increasing levels of the cytokinins and IAA, and decreasing levels of sucrose and starch in the shoot apex. Regeneration of new roots was preceded by an increase in the cytokinins and IAA, and a decrease in ABA in roots. Root sucrose increased dramatically one week after bud break and starch showed a general decreasing trend throughout the experiment.
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