University of Illinois Urbana-Champaign

Molecular genetics of Rhodobacter sphaeroides 2.4.1: Genome structure, plasmid characterization and chromosome transfer

Suwanto, Antonius

This item is only available for download by members of the University of Illinois community. Students, faculty, and staff at the U of I may log in with your NetID and password to view the item. If you are trying to access an Illinois-restricted dissertation or thesis, you can request a copy through your library's Inter-Library Loan office or purchase a copy directly from ProQuest.

Permalink

https://hdl.handle.net/2142/20426

Description

Title
Molecular genetics of Rhodobacter sphaeroides 2.4.1: Genome structure, plasmid characterization and chromosome transfer
Author(s)
Suwanto, Antonius
Issue Date
1992
Doctoral Committee Chair(s)
Salyers, Abigail A.
Department of Study
Microbiology
Discipline
Microbiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • Biology, Molecular
  • Biology, Genetics
  • Biology, Microbiology
Language
eng
Abstract
  • Physical mapping techniques were employed to construct both a physical and genetic maps of the Rhodobacter sphaeroides 2.4.1 genome. It was found that this $\alpha$-purple, nonsulfur photosynthetic bacterium carries two different circular chromosomes whose sizes are 3046 $\pm$ 95 and 914 $\pm$ 17 kb in addition to five endogenous plasmids with a total size of approximately 450 kb.
  • The smallest, 42 kb, endogenous plasmid was observed to be self transmissible. As little as an 425 bp oriT-containing DNA fragment isolated from this plasmid was able to confer transfer, between R. sphaeroides strains, when it was cloned into an otherwise non-transmissible plasmid. The DNA sequence of this oriT containing fragment reveals an A-T rich region, several direct and inverted repeats, as well as putative IHF-binding sites.
  • Random insertion of oriT into the R. sphaeroides 2.4.1 genome generated Hfr-like strains which exhibit a gradient of marker transfer, and has further provided classical genetic evidence for the circularity as well as the presence of two distinct chromosomal linkage groups in this bacterium.
Type of Resource
text
Permalink
http://hdl.handle.net/2142/20426
Copyright and License Information
Copyright 1992 Suwanto, Antonius

Owning Collections

Graduate Dissertations and Theses at Illinois PRIMARY
Graduate Theses and Dissertations at Illinois
Dissertations and Theses - Microbiology