Structure and expression of nuclear genes encoding light-harvesting chlorophyll a/b-binding polypeptides of photosystem II in apple and soybean
Chen, Houqi
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https://hdl.handle.net/2142/19991
Description
Title
Structure and expression of nuclear genes encoding light-harvesting chlorophyll a/b-binding polypeptides of photosystem II in apple and soybean
Author(s)
Chen, Houqi
Issue Date
1990
Doctoral Committee Chair(s)
Korban, Schuyler S.
Department of Study
Crop Sciences
Discipline
Crop Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Biology, Molecular
Biology, Genetics
Biology, Plant Physiology
Language
eng
Abstract
An apple genomic library was constructed with lambda Charon 35 vector. The library was screened using a pea LHCP-II cDNA and an apple LHCP-II gene (AB10) was identified, subcloned and characterized. AB10 contains an open reading frame of 807 nucleotides coding for a precursor of 269 amino acids divided by a transit peptide of 40 amino acids and a mature polypeptide of 229 amino acids. This gene also contains a TTGTTT-like polyadenylation signal, and a 5$\sp\prime$-promoter region including TATA-like and CACAT-like boxes. Comparisons between AB10 and other LHCP-II genes from various plant species indicate that all the LHCP-II genes have similarities, on the average, of about 70% at the nucleotide level and of about 80% at the amino acid level. The transit peptide sequence of AB10 differs from those of other LHCP-II genes of annual plants, suggesting more codon substitutions in AB10 transit peptide than silent base substitutions in those of the other LHCP-II genes.
It was found that there were two directly repeated DNA sequences, around 700 bp each, located in the 5$\sp\prime$ flanking region of the AB10 sequence. These repeated sequences are related to deletion of the insert DNA during subcloning. The deletion of the apple insert DNA was also relevant to recA-independent recombination system in E. coli strains.
The LHCP-II gene expression in a greening soybean cell culture was investigated. Regulation on expression of this gene is chloroplast development stage-specific, and the expression is under multiple controls, transcriptional and posttranscriptional.
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