Greenhouse, laser radiation, and in vitro culture studies on the growth and propagation of Algerian ivy (Hedera canariensis L.)
Al-Juboory, Karim Husain
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Permalink
https://hdl.handle.net/2142/19958
Description
Title
Greenhouse, laser radiation, and in vitro culture studies on the growth and propagation of Algerian ivy (Hedera canariensis L.)
Author(s)
Al-Juboory, Karim Husain
Issue Date
1990
Doctoral Committee Chair(s)
Williams, David J.
Department of Study
Crop Sciences
Discipline
Crop Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Agriculture, Plant Culture
Biology, Plant Physiology
Language
eng
Abstract
The results of this research demonstrated that the combination of benzylaminopurine (BA) + gibberellic acid (GA$\sb{4 + 7}$) (Promalin) promoted branching of Hedera canariensis (Algerian Ivy) better than applications of BA or GA$\sb{4 + 7}$ alone. Moreover, as the concentration of Atrinal is increased, branch length and leaf number significantly decreased for both pinched and unpinched plants. Plants treated with Atrinal displayed more new shoots per node than those treated with GA$\sb{4 + 7}$, BA, or Promalin. 2,3,5-triiodobenzoic acid (TIBA) significantly increased the branching of Hedera canariensis, although the shape of the plant was not commercially acceptable due to foliage epinasty.
Growth chamber studies on the effects of environmental conditions on the growth of Hedera canariensis showed that highest light intensity employed with a 12 hr photoperiod yielded the highest branch number at both 15.5$\sp\circ$C and 26.5$\sp\circ$C, as compared to other treatments. Similarly, total chlorophyll, chlorophyll a and chlorophyll b content increased with higher light intensity.
The use of laser technology in tissue culture was studied. In these studies, yttrium aluminum garnet (YAG) laser radiation with 60 and 230 mJ had no effect on the growth of Hedera canariensis explants.
More roots per explant developed on full strength Murashige and Skoog 1962 (MS) medium combined with naphthaleneacetic acid (NAA) plus light than with other treatments. With indolebutyric acid (IBA), the most roots per explant were obtained with a combination of IBA and 1/4 MS salt strength. There was an inverse relationship between an increase in IBA or NAA concentration and root length and root number. Activated charcoal supplemented with 1/4 MS salt strength significantly increased root number, root length, and rooting percentage more than the 1/2 and full MS salt strength treatments. Leaves cultured on MS medium containing low levels of Thidiazuron (TDZ) and high levels of NAA in the presence of light produced the highest number of roots. Pinching in vitro explants increased branch number at the lowest level of GA$\sb3$ + BA, but produced chlorotic plants similar to plants treated in the greenhouse with growth regulators.
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