Effects of in vivo dexamethasone sodium phosphate on in vitro equine neutrophil function
Sturtevant, Faye Coleman
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https://hdl.handle.net/2142/19795
Description
Title
Effects of in vivo dexamethasone sodium phosphate on in vitro equine neutrophil function
Author(s)
Sturtevant, Faye Coleman
Issue Date
1989
Department of Study
Veterinary Medicine - Pathobiology
Discipline
Pathobiology
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Agriculture, Animal Pathology
Language
eng
Abstract
Neutrophil function was evaluated in vitro 24 and 48 hours after administration of a single IV dose of dexamethasone sodium phosphate (0.088 mg/kg) to adult horses. Neutrophils were separated from whole blood using a two-step Percoll gradient. Purified neutrophils from dexamethasone-treated horses had impaired ability to kill Staphylococcus aureus as compared to pretreatment and normal horse neutrophils. Neither phagocytosis, iodination, or phagosome-lysosome fusion was affected by treatment. Hydrogen peroxide production, measured both spectrophotometrically and by flow cytometry, was not affected by treatment. Lysosomal enzyme activity for alkaline phosphatase, acid phosphatase, lysozyme, $\beta$-glucuronidase, and myeloperoxidase was quantitated and was not affected by treatment.
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