University of Illinois Urbana-Champaign

Initiation of translation of the small RNA of the cowpea strain of tobacco mosaic virus

Prouty, Rebecca E.

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Description

Title
Initiation of translation of the small RNA of the cowpea strain of tobacco mosaic virus
Author(s)
Prouty, Rebecca E.
Issue Date
1992
Doctoral Committee Chair(s)
Clark, John M., Jr.
Department of Study
Biochemistry
Discipline
Biochemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • Biology, Molecular
  • Chemistry, Biochemistry
Language
eng
Abstract
Several features of the initiation of translation of CcTMV-SRNA were investigated. First, the site of initiation of translation was found to be the first AUG in the 5$\sp\prime$-terminal sequence m$\sp7$GpppGUAUUUGAUGAUGGCA ... despite the fact that this AUG codon is not found within a favorable PuXXAUGG sequence. Second,analysis of the site of initiation of translation of mutant tCcTMV-SRNAs showed that the region of the tandem AUG's must contain some feature which directs the ribosome to initiate translation within that region and that the tandem AUGs themselves may be important for the actual selection of the correct translation initiator AUG codon. Third, investigation of the efficiency of the initiation of translation of the mutant tCcTMV-SRNAs showed that little if any relationship exists between the translational efficiency of the mutant tCcTMV-SRNAs and the agreement, of the sequences surrounding their translation initiation codons, with the PuXXAUGG consensus sequence. Finally, investigation of the translation of the mutant tCcTMV-SRNAs in the presence of $\sp{35}$SMet-tRNA$\sb{\rm i}\sp{\rm met}$ and $\sp{35}$S-Met-tRNA$\sb{\rm m}\sp{\rm met}$ produced unexpected results consistent with the miscoding of AAG codons to incorporate methionine under the conditions used.
Type of Resource
text
Permalink
http://hdl.handle.net/2142/19601
Copyright and License Information
Copyright 1992 Prouty, Rebecca E.

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Dissertations and Theses - Biochemistry