In vitro micropropagation, evaluation of disease resistance and hardiness of several new rose cultivars
Ameen, Sami Kareem
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https://hdl.handle.net/2142/19587
Description
Title
In vitro micropropagation, evaluation of disease resistance and hardiness of several new rose cultivars
Author(s)
Ameen, Sami Kareem
Issue Date
1989
Doctoral Committee Chair(s)
Meyer, Martin M.
Department of Study
Crop Sciences
Discipline
Crop Sciences
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
Agriculture, Plant Culture
Language
eng
Abstract
New hardy rose cultivars, R. hybrida 'Carefree Beauty', 'Prairie Youth' and 'John Frobisher' and R. rugosa 'Henry Hudson' and 'Jens Hunk' were successfully propagated in vitro. Stem pieces containing axillary buds, leaves and roots of these cultivars were the source of explants. Shoots obtained from axillary buds proliferated when they were cultured on a modified MS medium supplemented with combinations of BA and TDZ. Leaves and roots of 'Carefree Beauty', 'Prairie Youth' and 'John Frobisher' failed to regenerate shoots in vitro.
Histological studies of the proliferated shoots of 'Carefree Beauty', 'Prairie Youth' and 'John Frobisher' cultured in vitro indicated that there were no signs of any adventitious shoot initiation in any of the proliferating shoots.
Shoots of 'Carefree Beauty', 'Prairie Youth' and 'John Frobisher' failed to root when they were cultured on a modified MS medium only. Addition of NAA or IBA, AC to the medium stimulated rooting. When the MS salt strength was reduced to $1\over2$ or $1\over4$ with or without auxins in the medium, rooting was enhanced. An elevated sucrose concentration in the medium stimulated root initiation. Rooting of 'Carefree Beauty' and 'Prairie Youth' cuttings derived from in vitro micropropagated shoots was promoted if the cuttings were dipped in IBA solution before planting directly in soil.
Hardiness and disease resistance of seventeen new rose cultivars were studied. The observations of first year trials indicated that these cultivars were highly resistant to black spot, whereas, the growing season of 1988-1989 might not have been favorable for the powdery mildew infection. These newer roses were also able to survive the first cold winter of Illinois which killed the hybrid tea cultivars used for comparison.
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