Bioanalytical applications of microfluidic devices

Zhang, Huaibin

Permalink

https://hdl.handle.net/2142/16967 Copy

Description

Title

Bioanalytical applications of microfluidic devices

Author(s)

Zhang, Huaibin

Issue Date

2010-08-31T20:02:33Z

Director of Research (if dissertation) or Advisor (if thesis)

Nuzzo, Ralph G.

Doctoral Committee Chair(s)

Nuzzo, Ralph G.

Committee Member(s)

• Bailey, Ryan C.
• Gewirth, Andrew A.
• Sweedler, Jonathan V.

Department of Study

Chemistry

Discipline

Chemistry

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

• Microfluidic devices
• Patterning
• polyacrylamide hydrogel
• biomolecule
• Peptide
• protein
• polysaccharides
• microfluidic contact printing
• Deoxyribonucleic Acid (DNA)
• alkaline dehybridization
• single nucleotide polymorphisms (SNP)
• genotyping
• point-of-care
• isothermal genotyping
• stop-flow lithography
• kinetic discrimination

Abstract

The first part of the thesis describes a new patterning technique--microfluidic contact printing--that combines several of the desirable aspects of microcontact printing and microfluidic patterning and addresses some of their important limitations through the integration of a track-etched polycarbonate (PCTE) membrane. Using this technique, biomolecules (e.g., peptides, polysaccharides, and proteins) were printed in high fidelity on a receptor modified polyacrylamide hydrogel substrate. The patterns obtained can be controlled through modifications of channel design and secondary programming via selective membrane wetting. The protocols support the printing of multiple reagents without registration steps and fast recycle times. The second part describes a non-enzymatic, isothermal method to discriminate single nucleotide polymorphisms (SNPs). SNP discrimination using alkaline dehybridization has long been neglected because the pH range in which thermodynamic discrimination can be done is quite narrow. We found, however, that SNPs can be discriminated by the kinetic differences exhibited in the dehybridization of PM and MM DNA duplexes in an alkaline solution using fluorescence microscopy. We combined this method with multifunctional encoded hydrogel particle array (fabricated by stop-flow lithography) to achieve fast kinetics and high versatility. This approach may serve as an effective alternative to temperature-based method for analyzing unamplified genomic DNA in point-of-care diagnostic.

Graduation Semester

2010-08

Permalink

http://hdl.handle.net/2142/16967

Copyright and License Information

Copyright 2010 Huaibin Zhang

Owning Collections