Tools for modulating and measuring intracellular redox environment

Henderson, Jerrod A.

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https://hdl.handle.net/2142/16070 Copy

Description

Title

Tools for modulating and measuring intracellular redox environment

Author(s)

Henderson, Jerrod A.

Issue Date

2010-05-19T18:33:54Z

Director of Research (if dissertation) or Advisor (if thesis)

Kenis, Paul J.A.

Doctoral Committee Chair(s)

Kenis, Paul J.A.

Committee Member(s)

• Gaskins, H. Rex
• Kong, Hyun Joon
• Price, Nathan D.

Department of Study

Chemical & Biomolecular Engr

Discipline

Chemical Engineering

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

• redox environment
• glutathione
• porcine cells
• electrochemical platform

Abstract

Intracellular redox environment, the relative amount of oxidized and reduced chemical species within a cell, is important in regulating cellular processes; however, molecular mechanisms that mediate redox environment are poorly understood largely because of limitations of tools to both modulate and measure the intracellular redox environment. For example, pharmacological methods that modulate intracellular redox environment often lack selectivity, cause cellular toxicity, and perturb intracellular homeostasis. Methods for monitoring intracellular redox environment can also cause toxicity and protocols typically require disruption of cells. The overall goal of my dissertation research therefore was to develop and validate tools for both modulating and measuring the intracellular redox environment. This dissertation describes the development of three-electrode electrochemical platforms to modulate the intracellular redox environment of mammalian cells. The platforms are capable of both oxidizing and reducing the intracellular redox environment of Chinese hamster ovary (CHO) cells in correspondence to applied extracellular potential. I also demonstrate the utility of novel FRET-based biosensors, developed by collaborators, as real-time indicators of reduction/oxidation conditions in CHO and isogenic porcine fibroblasts. These studies will help unravel the role of redox regulation in many biological processes including those connected to normal and tumorigenic cells.

Graduation Semester

2010-5

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http://hdl.handle.net/2142/16070

Copyright and License Information

Copyright 2010 Jerrod A. Henderson

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