Studies of protein folding on membranes and in crowded environments and bridging the research-teaching gap in K-12 science

Denos, Sharlene

Permalink

https://hdl.handle.net/2142/14567 Copy

Description

Title

Studies of protein folding on membranes and in crowded environments and bridging the research-teaching gap in K-12 science

Author(s)

Denos, Sharlene

Issue Date

2010-01-06T16:12:35Z

Director of Research (if dissertation) or Advisor (if thesis)

Gruebele, Martin

Doctoral Committee Chair(s)

Martin Gruebele

Committee Member(s)

• Clegg, Robert M.
• Crofts, Antony R.
• Gennis, Robert B.

Department of Study

School of Molecular & Cell Bio

Discipline

Biophysics & Computnl Biology

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

• Proteins
• Protein Folding
• Crowding
• Science Education
• Science Outreach
• Membrane Proteins
• Peptide Design

Abstract

This work deals with three important problems in membrane protein folding studies, namely the preparation and storage of homogeneous small unilamellar vesicles (SUV), the development of an algorithm for selecting soluble trans-membrane helices from known membrane proteins, and the characterization of membrane binding of single surface and trans-membrane helices. I then describe the effects of excluded volume on the stability and kinetics of a stable Lambda Repressor mutant Y22WQ33YA3749G. Finally, I discuss two education projects that aim to bridge the gap between scientific research and K-12 teaching.

Graduation Semester

2009-12

Permalink

http://hdl.handle.net/2142/14567

Copyright and License Information

Copyright 2009 Sharlene Denos

Owning Collections