Effect of equipotent mixture of nitrate, atrazine and imidacloprid on gastrointestinal cell viability

Submitter: Beth Meschewski

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https://hdl.handle.net/2142/122947 Copy

Description

Title

Effect of equipotent mixture of nitrate, atrazine and imidacloprid on gastrointestinal cell viability

Contributor(s)

Chatman, Chamia

Issue Date

2024-04-24

Keyword(s)

Agricultural chemicals

Abstract

Livestock like humans may become exposed to complex mixtures of agricultural chemicals via oral ingestion of contaminated groundwater. Therefore, we aimed to elucidate the potential chemical-biological interactions and adverse outcome pathways associated with complex mixtures of agricultural chemicals detected in the 2021 Department of Agriculture, Trade and Consumer Protection’s (DATCP) Groundwater Quality Report. We determined that Portage, Waushara, Waupaca, and Pepin counties contained levels of atrazine (ATR), nitrate (NO3-), and imidacloprid (IMI) either above the enforcement standard or preventative action limit. However, chemical prioritization with toxEval indicated that only ATR had an exposure-activity ratio above the threshold of 0.001. Further evaluation of ATR, NO3- and IMI using checkerboard assays determined that combinations of 10 µg/mL of IMI + 10 µg/mL ATR and 10 µg/mL ATR + 1.25 µg/mL of NO3- causes the greatest growth inhibition to chicken cecal microbiome samples. In addition, Caco-2 cells exposed to this agricultural chemical mixture leads to decreased cell viability after a 24 h exposure compared to the equivalent mixture and each chemical singularly. In summary, the preliminary data suggests that an equipotent mixture leads to decreased cell viability after a 24 h exposure compared to the equivalent mixture and each chemical singularly.

Has Part

https://youtu.be/BfSvXFM5Neo

Type of Resource

• still image
• text

Language

eng

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