Discovery and characterization of RiPP natural products from radical SAM and lanthipeptide hybrid biosynthetic gene clusters
Frerk, Autumn M.
Loading…
Permalink
https://hdl.handle.net/2142/122000
Description
Title
Discovery and characterization of RiPP natural products from radical SAM and lanthipeptide hybrid biosynthetic gene clusters
Author(s)
Frerk, Autumn M.
Issue Date
2023-11-20
Director of Research (if dissertation) or Advisor (if thesis)
van der Donk, Wilfred A
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
RiPP
Lanthipeptide
Radical SAM
Biosynthetic gene clusters
FAST-RiPPs
Abstract
Ribosomally synthesized and post-translationally modified peptides are an abundant source of bioactive natural products. Using genome-mining techniques, new hybrid biosynthetic gene clusters have been discovered which contain the genes necessary for both lanthipeptide formation as well as modification on the precursor peptides by a radical SAM enzyme. Two examples of this type of gene cluster that are of particular interest to us are one from Scytonema hofmannii PCC 7110 and another from Butyrivibrio sp. VCD2006. Bioinformatic analyses were performed on these clusters, and they were coexpressed using the FAST-RiPPs method to characterize the modifications on the precursor peptides. The final natural products from these clusters could potentially have interesting bioactivities.
Use this login method if you
don't
have an
@illinois.edu
email address.
(Oops, I do have one)
IDEALS migrated to a new platform on June 23, 2022. If you created
your account prior to this date, you will have to reset your password
using the forgot-password link below.
