The distribution of antibiotics resistance in Champaign County
Zhang, Guorui
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Permalink
https://hdl.handle.net/2142/121377
Description
Title
The distribution of antibiotics resistance in Champaign County
Author(s)
Zhang, Guorui
Issue Date
2023-07-18
Director of Research (if dissertation) or Advisor (if thesis)
Nguyen, Thanh Huong
Department of Study
Civil & Environmental Eng
Discipline
Environ Engr in Civil Engr
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
M.S.
Degree Level
Thesis
Keyword(s)
antibiotic
resistance
distribution
Escherichia coli
MIC
Abstract
In the past few decades, antibiotics have been widely used to treat diseases in humans and animals. Previous studies have reported antibiotic resistance in various environments, posing serious public health concerns. This study aims to establish a profile of antibiotic resistance among communities with different characteristics in Champaign counties, IL. We isolated Escherichia coli (E. coli) from sewage samples from six sites in three towns A, B, and C. Town A site hosts an international community of college students who frequently travel. Town B is home to a community of permanent residents. Town C has a meat processing plant and also hosts the workers of this plant. We determined the minimum inhibitory concentration (MIC) with the microdilution method for the most commonly used antibiotics: tetracycline, ampicillin, ciprofloxacin, gentamicin, chloramphenicol, trimethoprim, and sulfamethoxazole. Among 36 sewage samples collected, 157 E coli isolates were isolated from 26 samples, and only 53 isolates showed resistance. We found that ampicillin resistance is common and found in all three towns, and their resistance percentages did not significantly differ between sampling sites. No isolates were found resistant to ciprofloxacin and gentamicin . Chloramphenicol resistance was only detected in the Meat Plant and one site of Town C (Town C3). The tetracycline-resistance percentages of the three sites in Town C were significantly greater than those of Town A and Town B but did not show significant differences between sites of Town C. Trimethoprim-sulfamethoxazole and trimethoprim had similar resistant percentages, mainly in Town C. Multiple-antibiotics resistance was detected in Town B and Town C, and Town C 3 had the highest multiple antibiotic resistance percentage. Comparing the percentage of ampicillin and tetracycline resistance, the peaks of tetracycline were found in May and August. Through antibiotic resistance was detected at high percentages in this study, we still need to test more E coli isolates at different sampling periods to better evaluate the antibiotic resistance in these communities. Future studies on phenotype by testing some other antibiotics and isolates for more sampling periods are required to better understand the characterization of community-level antibiotic resistance.
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