Protein corona formation and analysis on colloidal inorganic nanoparticles

Hoang, Khoi Nguyen Le

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https://hdl.handle.net/2142/121212 Copy

Description

Title

Protein corona formation and analysis on colloidal inorganic nanoparticles

Author(s)

Hoang, Khoi Nguyen Le

Issue Date

2023-07-10

Director of Research (if dissertation) or Advisor (if thesis)

Murphy, Catherine J

Doctoral Committee Chair(s)

Murphy, Catherine J

Committee Member(s)

• Chan, Jefferson
• Huang, Pinshane
• Wheeler, Korin E

Department of Study

Chemistry

Discipline

Chemistry

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

• Protein corona
• inorganic nanoparticles

Abstract

Due to their unique physiochemical properties exhibited at the nanoscale, nanoparticles are being increasingly utilized to better human experience in many different applications, including energy, transportation, information technology, medicine, food safety, and consumer products. The widespread interest and utilization of their tunable properties will likely result in the release of nanoparticles into the environment. Additional molecular understanding of the protein corona is needed to prevent negative environmental and health impacts and to develop sustainable nanotechnology. In this work, Chapter 1 introduces the field of nanotechnology and colloidal inorganic nanoparticles with a focus on gold and iron oxide nanoparticles as well as the concepts of the nanobio interface and the importance of the protein corona. Chapter 2 details the importance of isolation method on the protein corona composition when analyzing the protein corona upon incubation with gold-coated iron oxide nanoparticles. Chapter 3 describes the consequences of protein disulfide isomerase, a redox-active protein, upon exposure to gold nanoparticles using limited proteolytic mass spectrometry analysis. In Chapter 4, the focus is shifted to understanding the effects of nanoparticle surface functionalization on the protein corona formation in plants.

Graduation Semester

2023-08

Type of Resource

Thesis

Handle URL

https://hdl.handle.net/2142/121212

Copyright and License Information

Copyright 2023 Khoi Nguyen Hoang

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