University of Illinois Urbana-Champaign

Protein corona formation and analysis on colloidal inorganic nanoparticles

Hoang, Khoi Nguyen Le

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https://hdl.handle.net/2142/121212

Description

Title
Protein corona formation and analysis on colloidal inorganic nanoparticles
Author(s)
Hoang, Khoi Nguyen Le
Issue Date
2023-07-10
Director of Research (if dissertation) or Advisor (if thesis)
Murphy, Catherine J
Doctoral Committee Chair(s)
Murphy, Catherine J
Committee Member(s)
  • Chan, Jefferson
  • Huang, Pinshane
  • Wheeler, Korin E
Department of Study
Chemistry
Discipline
Chemistry
Degree Granting Institution
University of Illinois at Urbana-Champaign
Degree Name
Ph.D.
Degree Level
Dissertation
Keyword(s)
  • Protein corona
  • inorganic nanoparticles
Abstract
Due to their unique physiochemical properties exhibited at the nanoscale, nanoparticles are being increasingly utilized to better human experience in many different applications, including energy, transportation, information technology, medicine, food safety, and consumer products. The widespread interest and utilization of their tunable properties will likely result in the release of nanoparticles into the environment. Additional molecular understanding of the protein corona is needed to prevent negative environmental and health impacts and to develop sustainable nanotechnology. In this work, Chapter 1 introduces the field of nanotechnology and colloidal inorganic nanoparticles with a focus on gold and iron oxide nanoparticles as well as the concepts of the nanobio interface and the importance of the protein corona. Chapter 2 details the importance of isolation method on the protein corona composition when analyzing the protein corona upon incubation with gold-coated iron oxide nanoparticles. Chapter 3 describes the consequences of protein disulfide isomerase, a redox-active protein, upon exposure to gold nanoparticles using limited proteolytic mass spectrometry analysis. In Chapter 4, the focus is shifted to understanding the effects of nanoparticle surface functionalization on the protein corona formation in plants.
Graduation Semester
2023-08
Type of Resource
Thesis
Copyright and License Information
Copyright 2023 Khoi Nguyen Hoang

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