University of Illinois Urbana-Champaign

Assessing Pollinator Communities via Environmental DNA (eDNA) Metacommunity Assay.

Davis, Mark; Harper, L. R.; Molano-Flores, Brenda; Benito, J.; Niemiller, M. L.

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https://hdl.handle.net/2142/117181

Description

Title
Assessing Pollinator Communities via Environmental DNA (eDNA) Metacommunity Assay.
Author(s)
  • Davis, Mark
  • Harper, L. R.
  • Molano-Flores, Brenda
  • Benito, J.
  • Niemiller, M. L.
Issue Date
2020-06-04
Keyword(s)
  • Environmental DNA
  • Pollinators
  • Plant/Pollinator Communities
  • Metabarcoding primers
Abstract
Pollinators provide essential ecosystem services and are critical components of resilient ecosystems. Yet, pollinators are suffering staggering global declines leading to increased listing under the Endangered Species Act. This presents challenges for DoD installations. The technical objectives of this project were to: 1) develop and validate broad-spectrum pollinator eDNA metabarcoding primers, 2) test the efficacy of multiple capture methods to obtain Next Generation Sequencing-ready pollinator eDNA from flowers, and 3) validate eDNA sampling and microfluidic metabarcoding methods in controlled and field experiments with mixed plant/pollinator communities.
Publisher
Department of Defence Strategic Environmental Research and Development Progrram (SERDP)
Type of Resource
text
Language
en
Has Version(s)
NTIS National Technical Reports Library
Sponsor(s)/Grant Number(s)
Department of Defence Strategic Environmental Research and Development Progrram (SERDP) Project RC19-1102

Owning Collections

Staff Publications, Presentations, Reports, Etc. - Illinois Natural History Survey PRIMARY
Works by INHS staff that were not published by the INHS