Isolating The Intrinsic Spectral Responses Of Vibrational Probes: Benchmarks For Reporters Of Condensed Phase And Biological Processes

Edington, Sean Coleman

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https://hdl.handle.net/2142/116908 Copy

Description

Title

Isolating The Intrinsic Spectral Responses Of Vibrational Probes: Benchmarks For Reporters Of Condensed Phase And Biological Processes

Author(s)

Edington, Sean Coleman

Contributor(s)

• Johnson, Mark
• Mohamed, Ahmed

Issue Date

2022-06-22

Keyword(s)

Coblentz Special Session

Abstract

We use cryogenic infrared vibrational predissociation spectroscopy of isolated, nitrile-containing vibrational probe molecules to provide benchmarks for the probe molecule spectral response. Popular probes, such as paracyanophenylalanine, and other nitrile-containing molecules are manipulated in solution to modify conformation and charge state prior to extraction and isolation using electrospray ionization and He buffer gas cooling to ~10 K. The vibrational spectra of the cold, He- or H2-tagged molecules are collected in a linear predissociation regime and interpreted with the aid of electronic structure calculations. The results provide insight into the intrinsic spectral response of isolated nitrile vibrational reporters decoupled from solvent effects. Vibrational probe molecules are popularly employed to provide spectroscopic readouts of local electrostatic environments in phenomena including bulk solvation dynamics, interfacial proton transfer, and enzyme catalysis. A range of these molecules has been developed, allowing investigators to exploit absorption-free "windows" in the infrared spectrum to ease measurement. However, in the condensed phase it is often a challenge to separate the components of the reporter's response arising from external factors, such as local electric fields or hydrogen bonding, from those due to intrinsic factors such as molecular electrostatic potential or reporter isomerization.

Publisher

International Symposium on Molecular Spectroscopy

Type of Resource

text

Language

eng

Handle URL

https://hdl.handle.net/2142/116908

DOI

https://doi.org/10.15278/isms.2022.WE06

Copyright and License Information

Copyright 2022 held by the authors

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