Immunofluorescence of Mouse Hippocampus - Memory and Schizophrenia

Reyes, Jacob

Permalink

https://hdl.handle.net/2142/114205 Copy

Description

Title

Immunofluorescence of Mouse Hippocampus - Memory and Schizophrenia

Author(s)

Reyes, Jacob

Contributor(s)

Kambali, Maltesh

Issue Date

2022

Keyword(s)

Comparative Biosciences

Abstract

This is a three-strain merged image of the CA1 region of a mouse hippocampus. The three stains consist of glycine decarboxylase (GLDC) protein (red), the nucleus of cells such as neurons and astrocytes (blue), and astrocytes (green). The purpose of this merged immunofluorescent image was to observe any co-localization of GLDC protein and astrocytes in genetically modified mice (possessing a duplicate of the GLDC gene) to investigate the role of GLDC in astrocytes. GLDC is an enzyme that degrades glycine and may play a role in regulating NMDA receptor-dependent activity in the brain, specifically memory function in the hippocampus. The relevance of this protein is that it was found to be over-produced in individuals with schizophrenia. Rudolph Lab created the genetically modified mouse model of this overexpression so that we could understand how this overexpression affects memory through analyzing the hippocampus in experiments such as this. The knowledge gained from this research will hopefully be able to aid in making the lives of those with schizophrenia better through improved memory function.

Type of Resource

• Text
• Image

Language

eng

Permalink

http://hdl.handle.net/2142/114205

Copyright and License Information

Copyright 2022 Jacob Reyes

Owning Collections