Biological Controls for Standardization and Interpretation of Adaptive Immune Receptor Repertoire Profiling

Trück, Johannes; Eugster, Anne; Barennes, Pierre; Tipton, Christopher M.; Luning Prak, Eline T.; Bagnara, Davide; Soto, Cinque; Sherkow, Jacob S.; Payne, Aimee S.; Lefranc, Marie-Paule; Farmer, Andrew; The AIRR Community; Bostick, Magnolia; Mariotti-Ferrandiz, Encarnita

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https://hdl.handle.net/2142/110324 Copy

Description

Title

Biological Controls for Standardization and Interpretation of Adaptive Immune Receptor Repertoire Profiling

Author(s)

• Trück, Johannes
• Eugster, Anne
• Barennes, Pierre
• Tipton, Christopher M.
• Luning Prak, Eline T.
• Bagnara, Davide
• Soto, Cinque
• Sherkow, Jacob S.
• Payne, Aimee S.
• Lefranc, Marie-Paule
• Farmer, Andrew
• The AIRR Community
• Bostick, Magnolia
• Mariotti-Ferrandiz, Encarnita

Issue Date

2021-05-26

Keyword(s)

• B-cell Receptor (BCR)
• IG
• T-cell Receptor (TCR)
• TR
• antibody
• immunoglobulin
• immunology
• inflammation
• next generation sequencing (NGS)

Abstract

Use of adaptive immune receptor repertoire sequencing (AIRR-seq) has become widespread, providing new insights into the immune system with potential broad clinical and diagnostic applications. However, like many high-throughput technologies, it comes with several problems, and the AIRR Community was established to understand and help solve them. We, the AIRR Community's Biological Resources Working Group, have surveyed scientists about the need for standards and controls in generating and annotating AIRR-seq data. Here, we review the current status of AIRR-seq, provide the results of our survey, and based on them, offer recommendations for developing AIRR-seq standards and controls, including future work.

Publisher

eLife Sciences Publications

Type of Resource

text

Language

en

Permalink

http://hdl.handle.net/2142/110324

DOI

10.7554/eLife.66274

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