University of Illinois Urbana-Champaign

Using wax printing techniques to achieve multiplexing in paper-based lateral flow devices

Tung, Kevin

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https://hdl.handle.net/2142/110271

Description

Title
Using wax printing techniques to achieve multiplexing in paper-based lateral flow devices
Author(s)
Tung, Kevin
Contributor(s)
Zhao, Yang
Issue Date
2021-05
Keyword(s)
  • Lateral flow assay (LFA)
  • Point of Care (POC)
  • Paper-based lateral-flow assay
  • Wax printing
  • Multiplex
  • Paper-microfluidics
Abstract
With the recent emergence of the COVID-19 virus, the pandemic has affected the lives of millions of people and highlights the need for fast and accurate virus testing in order to further prevent the spread of this pandemic. Point of care testing has recently became one of the most popular diagnosis methods in clinical analysis due to its rapid test result times and low cost. One of the most widely used point of care technique is the paper-based lateral-flow assay, which is the focus of this thesis. One method to further reduce the cost of performing a paper-based lateral-flow test is the use of a Wax can be used to fabricate hydrophobic barriers on the nitrocellulose membrane of the lateral flow paper-based assay to create separate channels for different analytes to be detected simultaneously with the same amount of sample that was previously used to detect a single analyte. The multiplexing ability of the wax printed barriers helps greatly reduce the sample size and reagents needed to perform paper-based lateral-flow test. Here we show how we can apply wax printing techniques on paper-based lateral-flow assay to achieve multiplexing and further reduce the cost of operation.
Type of Resource
text
Language
en
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http://hdl.handle.net/2142/110271

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