Surface water collected at the Great Lake tributaries slightly activates nuclear hormone receptors of various species

Kohno, Satomi

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https://hdl.handle.net/2142/109881 Copy

Description

Title

Surface water collected at the Great Lake tributaries slightly activates nuclear hormone receptors of various species

Author(s)

Kohno, Satomi

Issue Date

2021-04-27

Keyword(s)

• emerging contaminants
• lake sturgeon
• hormones

Abstract

Presented by: Satomi Kohno – Assistant professor at St Cloud State University, skohno@stcloudstate.edu Co-authors: Heiko L. Schoenfuss Abstract: To date, researchers have mostly focused on model toxicological species such as Zebrafish or Medaka fish. However, we little know the impact of contaminants of emerging concern (CECs) on other aquatic organisms in the Great Lake Tributaries. Therefore, this project aims to develop and assess a multi-species trans-activation in vitro that evaluates species sensitivity differences to CECs without organismal approaches. This approach is useful, especially in endangered or threatened species. A long-read transcriptomic analysis identified 14 and 3 nuclear hormone receptors in hepatic and hepatopancreatic RNA in the Lake sturgeon and freshwater mussels, respectively. Lake Sturgeon significantly expressed estrogen receptor 1 (esr1) in gonad and the inferior lobe of the brain, androgen receptor (ar) in the gonad, and thyroid hormone receptor-β (thrb) in the cerebrum, eye, Inferior lobe, and olfactory bulb. We reproduce these expression profiles in a trans-activation assay in vitro so that we can reconstruct tissue-specific responses of nuclear hormone receptors. We also analyzed estrogen receptors in the Fathead minnow, Bluegill sunfish, Largemouth bass. Surface water collected at the Great Lake tributaries slightly activated these estrogen receptors. Further investigations on a diversity of receptors in various species for the risk and hazard assessments are required. Biography: Aquatic Toxicology Laboratory, Saint Cloud State University 720 Fourth Avenue South, Saint Cloud, MN 56301, United States; 320-200-9173; skohno@stcloudstate.edu

Series/Report Name or Number

2021 Emerging Contaminants in the Environment Conference (ECEC21)

Type of Resource

• text
• still image

Language

en

Permalink

• http://hdl.handle.net/2142/109881
• https://youtu.be/Ol6FoNX9tvc

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