Hard and soft coatings on gold nanoparticles at the nano-bio interface

Wu, Meng

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Description

Title

Hard and soft coatings on gold nanoparticles at the nano-bio interface

Author(s)

Wu, Meng

Issue Date

2020-05-04

Director of Research (if dissertation) or Advisor (if thesis)

Murphy, Catherine J.

Doctoral Committee Chair(s)

Murphy, Catherine J.

Committee Member(s)

• Chan, Jefferson
• Leal, Cecilia
• Lu, Yi

Department of Study

Chemistry

Discipline

Chemistry

Degree Granting Institution

University of Illinois at Urbana-Champaign

Degree Name

Ph.D.

Degree Level

Dissertation

Keyword(s)

Gold nanoparticles, NMR, ligand environment, protein corona.

Abstract

This dissertation explores surface chemistry of gold nanoparticles, with a special focus in developing analytical methods to decipher the environment of small molecule ligands and biomolecule coronas at the molecular level. Chapter 1 introduces the physical and chemical properties of gold nanoparticles and recent advancement on elucidating the surface chemistry and protein corona formation of gold nanorods. Chapter 2 and Chapter 3 probe nanoparticle systems at the molecular level with NMR techniques. Chapter 2 details the development of a solution NMR based approach, including chemical shift analysis, ligand density analysis, and T2 relaxation analysis, to explore the environment of a small molecule thiol ligand on gold nanospheres and gold nanorods. In Chapter 3, functional groups that are associated with the fluorescence origin and photobleaching of various carbon dot samples are identified by NMR spectroscopy. Chapter 4 describes how the silica porosity, silica thickness and ligand thickness impact the thermal reshaping behavior of silica coated gold nanorods. Chapter 5 explores the driven intermolecular forces in protein corona formation and the orientation of the protein corona on gold nanoparticles by an adapted BCA assay and limited proteolysis.

Graduation Semester

2020-05

Type of Resource

Thesis

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Copyright and License Information

Copyright 2020 Meng Wu

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